Mouse Cxcl16 (C-X-C motif chemokine 16) Sandwich ELISA (STJE0004395)

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STJE0004395

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This Cxcl16 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Cxcl16
Gene ID: 66102
Uniprot ID: CXL16_MOUSE
Specificity: This kit recognizes Mouse CXCL16 in samples. No significant cross-reactivity or interference between Mouse CXCL16 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Glycosylated.
Function Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. Also acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis.
Protein Name C-X-C Motif Chemokine 16
Scavenger Receptor For Phosphatidylserine And Oxidized Low Density Lipoprotein
Sr-Psox
Small-Inducible Cytokine B16
Transmembrane Chemokine Cxcl16
Database Links Reactome: R-MMU-380108
Reactome: -MMU-418594
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names C-X-C Motif Chemokine 16 ELISA kit
Scavenger Receptor For Phosphatidylserine And Oxidized Low Density Lipoprotein ELISA kit
Sr-Psox ELISA kit
Small-Inducible Cytokine B16 ELISA kit
Transmembrane Chemokine Cxcl16 ELISA kit
Cxcl16 ELISA kit
Srpsox ELISA kit
Specificity This kit recognizes Mouse CXCL16 in samples. No significant cross-reactivity or interference between Mouse CXCL16 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 99
EDTA plasma (n=8) 87-101 94
Cell culture media (n=8) 87-100 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 95.40 214.80 792.30 93.20 197.80 735.60
Standard deviation 6.50 11.00 39.60 6.40 11.70 33.80
CV (%) 6.81 5.12 5.00 6.87 5.92 4.59
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance