Mouse CTSS (Cathepsin S) Sandwich ELISA (STJE0019739)

SKU:
STJE0019739

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.54pg/mL
Detection Limit: 15.63-1000pg/mL
Short Description: This CTSS Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Ctss
Gene ID: 13040
Uniprot ID: CATS_MOUSE
Specificity: This kit recognizes Mouse CTSS in samples. No significant cross-reactivity or interference between Mouse CTSS and analogues was observed
Sample Type: Serum, plasma and other biological fluids
Function Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
Protein Name Cathepsin S
Database Links Reactome: R-MMU-1474228
Reactome: -MMU-1679131
Reactome: -MMU-2022090
Reactome: -MMU-2132295
Reactome: -MMU-6798695
Cellular Localisation Lysosome
Secreted
Cytoplasmic Vesicle
Phagosome
Alternative ELISA Names Cathepsin S ELISA kit
Ctss ELISA kit
Cats ELISA kit
Specificity This kit recognizes Mouse CTSS in samples. No significant cross-reactivity or interference between Mouse CTSS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-103 96
EDTA plasma (n=8) 89-104 95
Cell culture media (n=8) 94-106 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 46.14 133.71 432.95 48.79 128.28 402.01
Standard deviation 2.39 6.08 19.27 2.99 5.31 14.75
CV (%) 5.18 4.55 4.45 6.13 4.14 3.67
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance