Mouse CRP (C-Reactive Protein) Sandwich ELISA (STJE0019987)

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STJE0019987

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.18 ng/mL
Detection Limit: 0.39-25 ng/mL
Short Description: This CRP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Crp
Gene ID: 12944
Uniprot ID: CRP_MOUSE
Specificity: This kit recognizes Mouse CRP in samples. No significant cross-reactivity or interference between Mouse CRP and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Found in plasma.
Function Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Protein Name C-Reactive Protein
Database Links Reactome: R-MMU-173623
Cellular Localisation Secreted
Alternative ELISA Names C-Reactive Protein ELISA kit
Crp ELISA kit
Ptx1 ELISA kit
Specificity This kit recognizes Mouse CRP in samples. No significant cross-reactivity or interference between Mouse CRP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-100 92
EDTA plasma (n=8) 87-103 94
Cell culture media (n=8) 93-108 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.38 3.78 9.45 1.43 3.73 9.99
Standard deviation 0.09 0.21 0.41 0.10 0.21 0.53
CV (%) 6.54 5.48 4.34 6.79 5.64 5.34
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance