• Mouse COL3 (Collagen Type 3) Sandwich ELISA (STJE0004435)

Mouse COL3 (Collagen Type 3) Sandwich ELISA (STJE0004435)

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STJE0004435

£194.50 - £368.50
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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38ng/mL
Detection Limit: 15.63~1000ng/mL
Short Description: This COL3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Col3a1
Gene ID: 12825
Uniprot ID: CO3A1_MOUSE
Specificity: This kit recognizes Mouse COL3 in samples. No significant cross-reactivity or interference between Mouse COL3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Protein Name Collagen Alpha-1(Iii Chain
Database Links Reactome: R-MMU-1442490
Reactome: -MMU-1474244
Reactome: -MMU-1650814
Reactome: -MMU-186797
Reactome: -MMU-198933
Reactome: -MMU-2022090
Reactome: -MMU-216083
Reactome: -MMU-3000171
Reactome: -MMU-3000178
Reactome: -MMU-419037
Reactome: -MMU-8874081
Reactome: -MMU-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative ELISA Names Collagen Alpha-1(Iii Chain ELISA kit
Col3a1 ELISA kit
Specificity This kit recognizes Mouse COL3 in samples. No significant cross-reactivity or interference between Mouse COL3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 95
EDTA plasma (n=8) 93-109 101
Cell culture media (n=8) 93-109 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 47.70 150.12 451.77 46.59 157.32 451.48
Standard deviation 3.13 7.64 22.72 2.80 8.32 17.11
CV (%) 6.56 5.09 5.03 6.01 5.29 3.79
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance