• Mouse CGRP1 (Calcitonin Gene Related Peptide 1) Sandwich ELISA (STJE0004347)

Mouse CGRP1 (Calcitonin Gene Related Peptide 1) Sandwich ELISA (STJE0004347)

SKU:
STJE0004347

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This CGRP1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Calca
Gene ID: 12310
Uniprot ID: CALCA_MOUSE
Specificity: This kit recognizes Mouse CGRP1 in samples. No significant cross-reactivity or interference between Mouse CGRP1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.
Protein Name Calcitonin Gene-Related Peptide 1
Alpha-Type Cgrp
Calcitonin Gene-Related Peptide I
Cgrp-I
Database Links
Reactome: R-MMU-418555
Reactome: -MMU-419812
Cellular Localisation Secreted
Alternative ELISA Names Calcitonin Gene-Related Peptide 1 ELISA kit
Alpha-Type Cgrp ELISA kit
Calcitonin Gene-Related Peptide I ELISA kit
Cgrp-I ELISA kit
Calca ELISA kit
Calc ELISA kit
Specificity This kit recognizes Mouse CGRP1 in samples. No significant cross-reactivity or interference between Mouse CGRP1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-108 99
EDTA plasma (n=8) 93-104 98
Cell culture media (n=8) 89-103 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 51.60 91.70 452.77 49.79 93.25 428.03
Standard deviation 3.33 5.27 22.19 3.28 4.94 14.51
CV (%) 6.45 5.75 4.90 6.59 5.30 3.39
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance