| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This Ccl12 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Ccl12 |
| Gene ID: |
20293 |
| Uniprot ID: |
CCL12_MOUSE |
| Specificity: |
This kit recognizes Mouse CCL12/MCP-5 in samples. No significant cross-reactivity or interference between Mouse CCL12/MCP-5 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation. |
| Protein Name | C-C Motif Chemokine 12Mcp-1-Related ChemokineMonocyte Chemoattractant Protein 5Monocyte Chemotactic Protein 5Mcp-5Small-Inducible Cytokine A12 |
| Database Links | Reactome: R-MMU-380108 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-C Motif Chemokine 12 ELISA kitMcp-1-Related Chemokine ELISA kitMonocyte Chemoattractant Protein 5 ELISA kitMonocyte Chemotactic Protein 5 ELISA kitMcp-5 ELISA kitSmall-Inducible Cytokine A12 ELISA kitCcl12 ELISA kitMcp5 ELISA kitScya12 ELISA kit |
| Specificity | This kit recognizes Mouse CCL12/MCP-5 in samples. No significant cross-reactivity or interference between Mouse CCL12/MCP-5 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-99 | 93 |
| EDTA plasma (n=8) | 100-110 | 103 |
| Cell culture media (n=8) | 84-96 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 173.22 | 443.46 | 1632.31 | 168.36 | 471.43 | 1765.51 |
| Standard deviation | 9.16 | 20.67 | 72.47 | 8.96 | 22.72 | 91.63 |
| CV (%) | 5.29 | 4.66 | 4.44 | 5.32 | 4.82 | 5.19 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
