| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This C4a Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
C4b |
| Gene ID: |
12268 |
| Uniprot ID: |
CO4B_MOUSE |
| Specificity: |
This kit recognizes Mouse C4a in samples. No significant cross-reactivity or interference between Mouse C4a and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. |
| Protein Name | Complement C4-B Cleaved Into - Complement C4 Beta Chain - Complement C4 Alpha Chain - C4a Anaphylatoxin - Complement C4 Gamma Chain |
| Database Links | Reactome: R-MMU-166663Reactome: -MMU-174577Reactome: -MMU-381426Reactome: -MMU-8957275Reactome: -MMU-977606 |
| Cellular Localisation | SecretedSynapseCell ProjectionAxonDendrite |
| Alternative ELISA Names | Complement C4-B Cleaved Into - Complement C4 Beta Chain - Complement C4 Alpha Chain - C4a Anaphylatoxin - Complement C4 Gamma Chain ELISA kitC4b ELISA kitC4 ELISA kit |
| Specificity | This kit recognizes Mouse C4a in samples. No significant cross-reactivity or interference between Mouse C4a and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-107 | 101 |
| EDTA plasma (n=8) | 87-100 | 92 |
| Cell culture media (n=8) | 96-108 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 247.32 | 511.51 | 2112.16 | 245.30 | 535.88 | 2299.95 |
| Standard deviation | 16.62 | 21.79 | 87.87 | 16.36 | 26.20 | 99.59 |
| CV (%) | 6.72 | 4.26 | 4.16 | 6.67 | 4.89 | 4.33 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
