| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This mouse Bax kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Bax |
| Gene ID: |
12028 |
| Uniprot ID: |
BAX_MOUSE |
| Specificity: |
This kit recognizes Mouse Bax in samples. No significant cross-reactivity or interference between Mouse Bax and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in a wide variety of tissues. |
| Post Translational Modifications | Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12. |
| Function | Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production. |
| Protein Name | Apoptosis Regulator Bax |
| Database Links | Reactome: R-MMU-111457Reactome: -MMU-114294Reactome: -MMU-5620971Reactome: -MMU-6804114 |
| Cellular Localisation | Isoform Alpha: Mitochondrion Outer MembraneSingle-Pass Membrane ProteinCytoplasmNucleusColocalizes With 14-3-3 Proteins In The CytoplasmUnder Stress ConditionsUndergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion MembraneIsoform Beta: CytoplasmIsoform Gamma: Cytoplasm |
| Alternative CLIA Names | Apoptosis Regulator Bax CLIA kitBax CLIA kit |
| Specificity | This kit recognizes Mouse Bax in samples. No significant cross-reactivity or interference between Mouse Bax and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-101 | 96 |
| EDTA plasma(n=8) | 98-114 | 104 |
| Cell culture media(n=8) | 97-113 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 104.91 | 281.82 | 931.01 | 94.62 | 264.27 | 885.74 |
| Standard deviation | 11.09 | 22.71 | 77.74 | 7.59 | 23.92 | 89.64 |
| CV (%) | 10.57 | 8.06 | 8.35 | 8.02 | 9.05 | 10.12 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
