Recombinant Mouse Autotaxin/E-NPP2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100-140 kDa.

Mouse Autotaxin/E-NPP2 protein (Recombinant) (C-hFc) (STJP003046)

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STJP003046
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Host: HEK293 cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Formulation: Lyophilised from a 0.22 Mu m filtered solution of 20mM Tris, 150mM NaCl, pH 7.4
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method: <0.1 EU/Mu g of the protein by LAL method.
Gene Symbol: Enpp2
Gene ID: 18606
Uniprot ID: ENPP2_MOUSE
Immunogen Region: Ser49-Ile862
Immunogen: Recombinant Mouse Autotaxin/E-NPP2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser49-Ile862) of Mouse Autotaxin/E-NPP2 (Accession #NP_056559.2) fused with a His tag at the C-terminus.
Immunogen Sequence: QSSLTPLMVNGILGESVTLP LEFPAGEKVNFITWLFNETS LAFIVPHETKSPEIHVTNPK QGKRLNFTQSYSLQLSNLKM EDTGSYRAQISTKTSAKLSS YTLRILRQLRNIQVTNHSQL FQNMTCELHLTCSVEDADDN VSFRWEALGNTLSSQPNLTV SWDPRISSEQDYTCIAENAV SNLSFSVSAQKLCEDVKIQY TDTKM
Tissue Specificity Expressed in brain and adipose tissue.
Post Translational Modifications N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. Secretion requires simultaneous glycosylation on Asn-53 and Asn-410, while probable glycosylation of Asn-410 has a preferential role on lysoPLD activity. Not O-glycosylated. The interdomain disulfide bond between Cys-413 and Cys-805 is essential for catalytic activity.
Function Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. Its major substrate is lysophosphatidylcholine. Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF).
Protein Name Autotaxin
Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2
E-Npp 2
Extracellular Lysophospholipase D
Lysopld
Cellular Localisation Secreted
Alternative Protein Names Autotaxin protein
Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2 protein
E-Npp 2 protein
Extracellular Lysophospholipase D protein
Lysopld protein
Enpp2 protein
Npps2 protein
Pdnp2 protein

Information sourced from Uniprot.org

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