| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
20.34 pg/mL |
| Detection Limit: |
78.13-5000 pg/mL |
| Short Description: |
This ARG1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Arg1 |
| Gene ID: |
11846 |
| Uniprot ID: |
ARGI1_MOUSE |
| Specificity: |
This kit recognizes Mouse ARG1 in samples. No significant cross-reactivity or interference between Mouse ARG1 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in macrophages. Expressed in precursor and mature group 2 innate lymphoid cells (ILC2s). Expressed in lung tumor-associated myeloid cells. Expressed in lung tumor-infiltrating dendritic cells. |
| Function | Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively.the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. Plays a role in the immune response of alternatively activated or M2 macrophages in processes such as wound healing and tissue regeneration, immune defense against multicellular pathogens and parasites, and immune suppression and allergic inflammation.the regulatory outcome seems to be organ specific. In tumor-infiltrating dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs) plays a role in suppression of T cell-mediated antitumor immunity. |
| Protein Name | Arginase-1Liver-Type ArginaseType I Arginase |
| Database Links | Reactome: R-MMU-6798695Reactome: -MMU-70635 |
| Cellular Localisation | CytoplasmCytoplasmic Granule |
| Alternative ELISA Names | Arginase-1 ELISA kitLiver-Type Arginase ELISA kitType I Arginase ELISA kitArg1 ELISA kit |
| Specificity | This kit recognizes Mouse ARG1 in samples. No significant cross-reactivity or interference between Mouse ARG1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-97 | 91 |
| EDTA plasma (n=8) | 91-107 | 99 |
| Cell culture media (n=8) | 84-98 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 331.49 | 926.71 | 3243.72 | 340.78 | 882.40 | 2996.82 |
| Standard deviation | 19.06 | 46.80 | 148.56 | 18.81 | 47.12 | 151.34 |
| CV (%) | 5.75 | 5.05 | 4.58 | 5.52 | 5.34 | 5.05 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
