Mouse Apoa1 (Apolipoprotein A-I) Sandwich ELISA (STJE0005332)

SKU:
STJE0005332

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 0.63~40ng/mL
Short Description: This Apoa1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Apoa1
Gene ID: 11806
Uniprot ID: APOA1_MOUSE
Specificity: This kit recognizes Mouse ApoA1 in samples. No significant cross-reactivity or interference between Mouse ApoA1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Glycosylated. Palmitoylated. May be acylated. Phosphorylation sites are present in the extracellular medium.
Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Protein Name Apolipoprotein A-I
Apo-Ai
Apoa-I
Apolipoprotein A1 Cleaved Into - Proapolipoprotein A-I
Proapoa-I - Truncated Apolipoprotein A-I
Database Links Reactome: R-MMU-114608
Reactome: -MMU-1369062
Reactome: -MMU-2168880
Reactome: -MMU-3000471
Reactome: -MMU-3000480
Reactome: -MMU-381426
Reactome: -MMU-8957275
Reactome: -MMU-8963888
Reactome: -MMU-8963896
Reactome: -MMU-8963901
Reactome: -MMU-8964011
Reactome: -MMU-8964058
Reactome: -MMU-9707616
Reactome: -MMU-975634
Cellular Localisation Secreted
Alternative ELISA Names Apolipoprotein A-I ELISA kit
Apo-Ai ELISA kit
Apoa-I ELISA kit
Apolipoprotein A1 Cleaved Into - Proapolipoprotein A-I ELISA kit
Proapoa-I - Truncated Apolipoprotein A-I ELISA kit
Apoa1 ELISA kit
Specificity This kit recognizes Mouse ApoA1 in samples. No significant cross-reactivity or interference between Mouse ApoA1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-108 103
EDTA plasma (n=8) 83-97 91
Cell culture media (n=8) 101-111 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.92 4.49 19.70 1.93 4.28 16.05
Standard deviation 0.11 0.21 0.91 0.10 0.20 0.73
CV (%) 5.73 4.68 4.62 5.18 4.67 4.55
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance