• Mouse ANG (Angiogenin) CLIA Kit (STJC0001061)

Mouse ANG (Angiogenin) CLIA Kit (STJC0001061)

SKU:
STJC0001061

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Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This mouse ANG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Ang
Gene ID: 11727
Uniprot ID: ANGI_MOUSE
Specificity: This kit recognizes Mouse ANG in samples. No significant cross-reactivity or interference between Mouse ANG and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Ribonuclease that cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Binds to actin on the surface of endothelial cells.once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
Protein Name Angiogenin
Angiogenin-1
Ribonuclease 5
Rnase 5
Database Links Reactome: R-MMU-418990
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle Lumen
Secreted
Nucleus
Nucleolus
Rapidly Endocytosed By Target Cells And Translocated To The Nucleus Where It Accumulates In The Nucleolus And Binds To Dna
Alternative CLIA Names Angiogenin CLIA kit
Angiogenin-1 CLIA kit
Ribonuclease 5 CLIA kit
Rnase 5 CLIA kit
Ang CLIA kit
Ang1 CLIA kit
Rnase5 CLIA kit
Rnase5a CLIA kit
Specificity This kit recognizes Mouse ANG in samples. No significant cross-reactivity or interference between Mouse ANG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-107 99
EDTA plasma(n=8) 90-105 97
Cell culture media(n=8) 88-101 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 53.82 155.34 432.63 54.74 149.29 449.57
Standard deviation 5.64 13.75 32.97 6.43 11.14 47.56
CV (%) 10.48 8.85 7.62 11.75 7.46 10.58
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance