• Mouse Ang-2 (Angiotensin 2) Competitive ELISA (STJE0005235)

Mouse Ang-2 (Angiotensin 2) Competitive ELISA (STJE0005235)

SKU:
STJE0005235

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This Ang-2 Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: AgtUniprot ID=ANGT_MOUSE"
Specificity: This kit recognizes Mouse Ang-2 in samples. No significant cross-reactivity or interference between Mouse Ang-2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2: Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2. Also binds the DEAR/FBXW7-AS1 receptor. Angiotensin-3: Stimulates aldosterone release. Angiotensin 1-7: Is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
Protein Name Angiotensinogen
Serpin A8 Cleaved Into - Angiotensin-1
Angiotensin 1-10
Angiotensin I
Ang I - Angiotensin-2
Angiotensin 1-8
Angiotensin Ii
Ang Ii - Angiotensin-3
Angiotensin 2-8
Angiotensin Iii
Ang Iii
Des-Asp1-Angiotensin Ii - Angiotensin-4
Angiotensin 3-8
Angiotensin Iv
Ang Iv - Angiotensin 1-9 - Angiotensin 1-7 - Angiotensin 1-5 - Angiotensin 1-4
Cellular Localisation Secreted
Alternative ELISA Names Angiotensinogen ELISA kit
Serpin A8 Cleaved Into - Angiotensin-1 ELISA kit
Angiotensin 1-10 ELISA kit
Angiotensin I ELISA kit
Ang I - Angiotensin-2 ELISA kit
Angiotensin 1-8 ELISA kit
Angiotensin Ii ELISA kit
Ang Ii - Angiotensin-3 ELISA kit
Angiotensin 2-8 ELISA kit
Angiotensin Iii ELISA kit
Ang Iii ELISA kit
Des-Asp1-Angiotensin Ii - Angiotensin-4 ELISA kit
Angiotensin 3-8 ELISA kit
Angiotensin Iv ELISA kit
Ang Iv - Angiotensin 1-9 - Angiotensin 1-7 - Angiotensin 1-5 - Angiotensin 1-4 ELISA kit
Agt ELISA kit
Serpina8 ELISA kit
Specificity This kit recognizes Ang-Ⅱ in samples. No significant cross-reactivity or interference between Ang-Ⅱ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-105 100
EDTA plasma(n=8) 85-101 92
Cell culture media(n=8) 86-100 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 46.00 138.00 442.80 42.50 130.80 407.70
Standard deviation 2.40 8.10 22.10 2.70 6.90 17.50
CV (%) 5.22 5.87 4.99 6.35 5.28 4.29
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance