| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This Alox15 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Alox15 |
| Gene ID: |
11687 |
| Uniprot ID: |
LOX15_MOUSE |
| Specificity: |
This kit recognizes Mouse 15-LO in samples. No significant cross-reactivity or interference between Mouse 15-LO and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. It inserts peroxyl groups at C12 or C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing both 12-hydroperoxyeicosatetraenoate/12-HPETE and 15-hydroperoxyeicosatetraenoate/15-HPETE. It may then act on 12-HPETE to produce hepoxilins, which may show pro-inflammatory properties. Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate. May participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs)like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively down-regulate the immune response and have anti-aggregation properties with platelets. Can convert epoxy fatty acids to hydroperoxy-epoxides derivatives followed by an intramolecular nucleophilic substitution leading to the formation of monocyclic endoperoxides. Plays an important role during the maintenance of self-tolerance by peroxidizing membrane-bound phosphatidylethanolamine which can then signal the sorting process for clearance of apoptotic cells during inflammation and prevent an autoimmune response. In addition to its role in the immune and inflammatory responses, this enzyme may play a role in epithelial wound healing in the cornea through production of lipoxin A4 (LXA(4)) and docosahexaenoic acid-derived neuroprotectin D1 (NPD1.10R,17S-HDHA), both lipid autacoids exhibit anti-inflammatory and neuroprotective properties. Furthermore, it may regulate actin polymerization which is crucial for several biological processes such as the phagocytosis of apoptotic cells. It is also implicated in the generation of endogenous ligands for peroxisome proliferator activated receptor (PPAR-gamma), hence modulating macrophage development and function. It may also exert a negative effect on skeletal development by regulating bone mass through this pathway. As well as participates in ER stress and downstream inflammation in adipocytes, pancreatic islets, and liver. Finally, it is also involved in the cellular response to IL13/interleukin-13. |
| Protein Name | Polyunsaturated Fatty Acid Lipoxygenase Alox1512/15-Lipoxygenase12/15-LoArachidonate 12-Lipoxygenase - Leukocyte-Type12-LoxL-12loArachidonate 15-Lipoxygenase15-LoxArachidonate Omega-6 LipoxygenaseHepoxilin A3 Synthase Alox15Linoleate 13s-Lipoxygenase |
| Database Links | Reactome: R-MMU-2142691Reactome: -MMU-2142712Reactome: -MMU-2142770Reactome: -MMU-9018677Reactome: -MMU-9018681Reactome: -MMU-9018896Reactome: -MMU-9023661Reactome: -MMU-9025106Reactome: -MMU-9026286 |
| Cellular Localisation | CytoplasmCytosolCell MembranePeripheral Membrane ProteinLipid DropletPredominantly CytosolicBecomes Enriched At Membranes Upon Calcium BindingTranslocates From The Cytosol To The Plasma Membrane When Stimulated By Il13/Interleukin-13 And In Macrophages Binding Apoptotic Cells |
| Alternative ELISA Names | Polyunsaturated Fatty Acid Lipoxygenase Alox15 ELISA kit12/15-Lipoxygenase ELISA kit12/15-Lo ELISA kitArachidonate 12-Lipoxygenase - Leukocyte-Type ELISA kit12-Lox ELISA kitL-12lo ELISA kitArachidonate 15-Lipoxygenase ELISA kit15-Lox ELISA kitArachidonate Omega-6 Lipoxygenase ELISA kitHepoxilin A3 Synthase Alox15 ELISA kitLinoleate 13s-Lipoxygenase ELISA kitAlox15 ELISA kitAlox12l ELISA kit |
| Specificity | This kit recognizes Mouse 15-LO in samples. No significant cross-reactivity or interference between Mouse 15-LO and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-109 | 101 |
| EDTA plasma (n=8) | 94-110 | 101 |
| Cell culture media (n=8) | 90-103 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 99.38 | 261.45 | 957.96 | 97.94 | 249.79 | 976.99 |
| Standard deviation | 5.63 | 15.50 | 37.94 | 6.81 | 12.51 | 32.83 |
| CV (%) | 5.67 | 5.93 | 3.96 | 6.95 | 5.01 | 3.36 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
