| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.051ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This Alk-Smase Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Enpp7Uniprot ID=ENPP7_MOUSE" |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in liver and small intestine. |
| Post Translational Modifications | N-glycosylated.required for activity and transport to the plasma membrane. |
| Function | Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract. Has also phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation. Does not have nucleotide pyrophosphatase activity. May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen. |
| Protein Name | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 7E-Npp 7Npp-7Alkaline Sphingomyelin PhosphodiesteraseIntestinal Alkaline SphingomyelinaseAlk-Smase |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinThe Catalytic Domain Is Released Into The Extracellular Medium When Cells Are Treated With TrypsinLocalized At The Surface Of The Microvillar Membrane In Small Intestine EnterocytesAnd In Endosome-Like Structures Situated Beneath The Microvillar MembraneAnd In Golgi Complex |
| Alternative ELISA Names | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 7 ELISA kitE-Npp 7 ELISA kitNpp-7 ELISA kitAlkaline Sphingomyelin Phosphodiesterase ELISA kitIntestinal Alkaline Sphingomyelinase ELISA kitAlk-Smase ELISA kitEnpp7 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
