Mouse Adiponectin/Acrp30/ADIPOQ protein (Recombinant) (C-His) (STJP001459)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse Adiponectin/Acrp30/ADIPOQ Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu18-Asn247) of mouse Adiponectin/Acrp30 (Accession #NP_033735.3) fused with a 6×His tag at the C-termi
General Information
| Short Description | Recombinant-Mouse Adiponectin/Acrp30/ADIPOQ-C-His protein was developed in hek293 cells using the region Glu18-Asn247. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 Mu g/mL (100 Mu L/well) can bind Human TNFRSF12A with a linear range of 0.1-2.3 ng/mL. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | Adipoq |
| Gene ID | 11450 |
| Uniprot ID | ADIPO_MOUSE |
| Immunogen | Recombinant Mouse Adiponectin/Acrp30/ADIPOQ Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu18-Asn247) of mouse Adiponectin/Acrp30 (Accession #NP_033735.3) fused with a 6×His tag at the C-termi |
| Immunogen Region | Glu18-Asn247 |
| Immunogen Sequence | EDDVTTTEELAPALVPPPKG TCAGWMAGIPGHPGHNGTPG RDGRDGTPGEKGEKGDAGLL GPKGETGDVGMTGAEGPRGF PGTPGRKGEPGEAAYVYRSA FSVGLETRVTVPNVPIRFTK IFYNQQNHYDGSTGKFYCNI PGLYYFSYHITVYMKDVKVS LFKKDKAVLFTYDQYQEKNV DQASGSVLLHLEVGDQVWLQ VYGDGDHNGLYADNVNDSTF TGFLLYHDTN |
Additional Info
| Post Translational Modifications | HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagen-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated O-linked glycans on hydroxylysine residues consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. O-linked glycosylation in the N-terminal is disialylated with the structure Neu5Acalpha2->8Neu5Acalpha2->3Gal. Sialylated by alpha 2,8-sialyltransferase III. Succination of Cys-39 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits polymerization and secretion of adiponectin. Adiponectin is a major target for succination in both adipocytes and adipose tissue of diabetic mice. It was proposed that succination of proteins is a biomarker of mitochondrial stress and accumulation of Krebs cycle intermediates in adipose tissue in diabetes and that succination of adiponectin may contribute to the decrease in plasma adiponectin in diabetes. |
| Function | Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. |
| Protein Name | Adiponectin30 Kda Adipocyte Complement-Related ProteinAdipocyte Complement-Related 30 Kda ProteinAcrp30Adipocyte - C1q And Collagen Domain-Containing ProteinAdipocyte-Specific Protein Adipoq |
| Database Links | Reactome: R-MMU-163680 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Adiponectin protein30 Kda Adipocyte Complement-Related Protein proteinAdipocyte Complement-Related 30 Kda Protein proteinAcrp30 proteinAdipocyte - C1q And Collagen Domain-Containing Protein proteinAdipocyte-Specific Protein Adipoq proteinAdipoq proteinAcdc proteinAcrp30 proteinApm1 protein |
Information sourced from Uniprot.org