| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.055ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This ABCG2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Abcg2 |
| Gene ID: |
26357 |
| Uniprot ID: |
ABCG2_MOUSE |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Highly expressed in kidney. Lower expression in liver, colon, heart, spleen, and placenta. Expressed in mammary gland. Expressed in intestinal villi and renal proximal tubules, hepatic bile canalicular membranes, and placental labyrinth cells (at protein level). |
| Post Translational Modifications | N-glycosylated. Glycosylation-deficient ABCG2 is normally expressed and functional. Phosphorylated. Phosphorylation may regulate the localization to the plasma membrane, the homooligomerization and therefore, the activity of the transporter. |
| Function | Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme. Also mediates the efflux of sphingosine-1-P from cells. Acts as a urate exporter functioning in both renal and extrarenal urate excretion. In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate. Also involved in the excretion of steroids like estrone 3-sulfate/E1S, 3beta-sulfooxy-androst-5-en-17-one/DHEAS, and other sulfate conjugates. Mediates the secretion of the riboflavin and biotin vitamins into milk. Extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability. Plays an important role in the exclusion of xenobiotics from the brain. It confers to cells a resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin, through the control of their efflux. In placenta, it limits the penetration of drugs from the maternal plasma into the fetus. May play a role in early stem cell self-renewal by blocking differentiation (Probable). |
| Protein Name | Broad Substrate Specificity Atp-Binding Cassette Transporter Abcg2Atp-Binding Cassette Sub-Family G Member 2Breast Cancer Resistance Protein 1 HomologUrate ExporterCd Antigen Cd338 |
| Database Links | Reactome: R-MMU-189451Reactome: -MMU-189483Reactome: -MMU-917937Reactome: -MMU-9753281Reactome: -MMU-9793528 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinApical Cell MembraneMitochondrion MembraneEnriched In Membrane Lipid Rafts |
| Alternative ELISA Names | Broad Substrate Specificity Atp-Binding Cassette Transporter Abcg2 ELISA kitAtp-Binding Cassette Sub-Family G Member 2 ELISA kitBreast Cancer Resistance Protein 1 Homolog ELISA kitUrate Exporter ELISA kitCd Antigen Cd338 ELISA kitAbcg2 ELISA kitAbcp ELISA kitBcrp1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
