| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | MAPK4 Positive Control for STJ501706 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: | WB: 1:500 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | MAPK4 |
| Gene ID: | 5596 |
| Uniprot ID: | MK04_HUMAN |
| Specificity: | This is positive control is recommended for use in combination with MAPK4 antibody STJ501706. |
| Tissue Specificity | High expression in heart and brain. |
| Post Translational Modifications | Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites. |
| Function | Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle. |
| Peptide Name | Mitogen-Activated Protein Kinase 4 Map Kinase 4 Mapk 4 Extracellular Signal-Regulated Kinase 4 Erk-4 Map Kinase Isoform P63 P63-Mapk |
| Database Links | Reactome: R-HSA-5687128 |
| Cellular Localisation | Cytoplasm Nucleus Translocates To The Cytoplasm Following Interaction With Mapkapk5 |
| Alternative Peptide Names | Mitogen-Activated Protein Kinase 4 protein Map Kinase 4 protein Mapk 4 protein Extracellular Signal-Regulated Kinase 4 protein Erk-4 protein Map Kinase Isoform P63 protein P63-Mapk protein MAPK4 protein ERK4 protein PRKM4 protein |
Information sourced from Uniprot.org