| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
MAP2K1 Blocking Peptide for STJ501657 is synthetically produced from the 300-350 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
MAP2K1 |
| Gene ID: |
5604 |
| Uniprot ID: |
MP2K1_HUMAN |
| Immunogen Region: |
300-350 |
| Specificity: |
This blocking peptide is recommended for use in combination with MAP2K1 antibody, STJ501657 |
| Immunogen: |
Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase kinase 1. |
| Tissue Specificity | Widely expressed, with extremely low levels in brain. |
| Post Translational Modifications | Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation. (Microbial infection) Acetylation by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner.by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. |
| Peptide Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 1Map Kinase Kinase 1Mapkk 1Mkk1Erk Activator Kinase 1Mapk/Erk Kinase 1Mek 1 |
| Database Links | Reactome: R-HSA-110056Reactome: R-HSA-170968Reactome: R-HSA-445144Reactome: R-HSA-5210891Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-5684264Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9652169Reactome: R-HSA-9656223 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle Pole BodyNucleusMembranePeripheral Membrane ProteinLocalizes At Centrosomes During PrometaphaseMidzone During Anaphase And Midbody During Telophase/CytokinesisMembrane Localization Is Probably Regulated By Its Interaction With Ksr1 |
| Alternative Peptide Names | Dual Specificity Mitogen-Activated Protein Kinase Kinase 1 proteinMap Kinase Kinase 1 proteinMapkk 1 proteinMkk1 proteinErk Activator Kinase 1 proteinMapk/Erk Kinase 1 proteinMek 1 proteinMAP2K1 proteinMEK1 proteinPRKMK1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
