| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
LIMK1 Blocking Peptide for STJ501610 is synthetically produced from the 550-647 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
LIMK1 |
| Gene ID: |
3984 |
| Uniprot ID: |
LIMK1_HUMAN |
| Immunogen Region: |
550-647 |
| Specificity: |
This blocking peptide is recommended for use in combination with LIMK1 antibody, STJ501610 |
| Immunogen: |
Synthetic peptide taken within amino acid region 550-647 on human LIM Kinase 1 protein. |
| Tissue Specificity | Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. |
| Post Translational Modifications | Autophosphorylated. Phosphorylated on Thr-508 by ROCK1 and PAK1, resulting in activation. Phosphorylated by PAK4 which increases the ability of LIMK1 to phosphorylate cofilin. Phosphorylated at Ser-323 by MAPKAPK2 during activation of VEGFA-induced signaling, which results in activation of LIMK1 and promotion of actin reorganization, cell migration, and tubule formation of endothelial cells. Dephosphorylated and inactivated by SSH1. Phosphorylated by CDC42BP. Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. |
| Function | Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3: Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1). |
| Peptide Name | Lim Domain Kinase 1Limk-1 |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-3928662Reactome: R-HSA-399954Reactome: R-HSA-416572Reactome: R-HSA-5627117Reactome: R-HSA-5627123 |
| Cellular Localisation | CytoplasmNucleusCytoskeletonCell ProjectionLamellipodiumPredominantly Found In The CytoplasmLocalizes In The Lamellipodium In A Cdc42bpaCdc42bpb And Fam89b/Lrap25-Dependent Manner |
| Alternative Peptide Names | Lim Domain Kinase 1 proteinLimk-1 proteinLIMK1 proteinLIMK protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
