IRE1 Blocking Peptide peptide (STJ504267)

SKU:
STJ504267-250

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Applications: Immunodepletion/Immunocompetition
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: IRE1 Blocking Peptide is synthetically produced from the 900-1000 sequence and is suitable for use in western blot applications.
Formulation: Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: ERN1
Gene ID: 2081
Uniprot ID: ERN1_HUMAN
Immunogen Region: 900-1000
Immunogen: Synthetic peptide corresponding to unique amino acid sequence within amino acid region 900-1000 on human IRE1A protein.
Tissue Specificity Ubiquitously expressed. High levels observed in pancreatic tissue.
Post Translational Modifications Autophosphorylated following homodimerization. Autophosphorylation promotes activation of the endoribonuclease domain. In response to ER stress, phosphorylated at Ser-724, Ser-729 and possibly Ser-726.phosphorylation promotes oligomerization and endoribonuclease activity. Dephosphorylated at Ser-724, Ser-729 and possibly Ser-726 by RPAP2 to abort failed ER-stress adaptation and trigger apoptosis. ADP-ribosylated by PARP16 upon ER stress, which increases both kinase and endonuclease activities.
Function Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes. Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A.
Peptide Name Serine/Threonine-Protein Kinase/Endoribonuclease Ire1
Endoplasmic Reticulum-To-Nucleus Signaling 1
Inositol-Requiring Protein 1
Hire1p
Ire1-Alpha
Ire1a Includes - Serine/Threonine-Protein Kinase - Endoribonuclease
Database Links Reactome: R-HSA-381070
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Alternative Peptide Names Serine/Threonine-Protein Kinase/Endoribonuclease Ire1 protein
Endoplasmic Reticulum-To-Nucleus Signaling 1 protein
Inositol-Requiring Protein 1 protein
Hire1p protein
Ire1-Alpha protein
Ire1a Includes - Serine/Threonine-Protein Kinase - Endoribonuclease protein
ERN1 protein
IRE1 protein

Information sourced from Uniprot.org

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