| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.062ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This VRK1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
VRK1 |
| Gene ID: |
7443 |
| Uniprot ID: |
VRK1_HUMAN |
| Sample Type: |
serum, plasma or other biological fluids. |
| Tissue Specificity | Widely expressed. Highly expressed in fetal liver, testis and thymus. |
| Post Translational Modifications | Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53. Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis. |
| Function | Serine/threonine kinase involved in cell cycle, nuclear condensation and transcription regulation. Involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity. |
| Protein Name | Serine/Threonine-Protein Kinase Vrk1Vaccinia-Related Kinase 1 |
| Database Links | Reactome: R-HSA-2980766Reactome: R-HSA-2995383 |
| Cellular Localisation | NucleusCytoplasmDispersed Throughout The Cell But Not Located On Mitotic Spindle Or Chromatids During Mitosis |
| Alternative ELISA Names | Serine/Threonine-Protein Kinase Vrk1 ELISA kitVaccinia-Related Kinase 1 ELISA kitVRK1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
