| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88ng/mL |
| Detection Limit: |
3.13~200ng/mL |
| Short Description: |
This VLDLR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
VLDLR |
| Gene ID: |
7436 |
| Uniprot ID: |
VLDLR_HUMAN |
| Specificity: |
This kit recognizes Human VLDLR in samples. No significant cross-reactivity or interference between Human VLDLR and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Ubiquitinated at Lys-839 by MYLIP leading to degradation. Glycosylated. |
| Function | Multifunctional cell surface receptor that binds VLDL and transports it into cells by endocytosis and therefore plays an important role in energy metabolism. Binds also to a wide range of other molecules including Reelin/RELN or apolipoprotein E/APOE-containing ligands as well as clusterin/CLU. In the off-state of the pathway, forms homooligomers or heterooligomers with LRP8. Upon binding to ligands, homooligomers are rearranged to higher order receptor clusters that transmit the extracellular RELN signal to intracellular signaling processes by binding to DAB1. This interaction results in phosphorylation of DAB1 leading to the ultimate cell responses required for the correct positioning of newly generated neurons. Later, mediates a stop signal for migrating neurons, preventing them from entering the marginal zone. (Microbial infection) Acts as a receptor for Semliki Forest virus. |
| Protein Name | Very Low-Density Lipoprotein ReceptorVldl ReceptorVldl-R |
| Database Links | Reactome: R-HSA-8866376Reactome: R-HSA-8866427Reactome: R-HSA-8964046 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembraneClathrin-Coated Pit |
| Alternative ELISA Names | Very Low-Density Lipoprotein Receptor ELISA kitVldl Receptor ELISA kitVldl-R ELISA kitVLDLR ELISA kit |
| Specificity | This kit recognizes Human VLDLR in samples. No significant cross-reactivity or interference between Human VLDLR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-106 | 100 |
| EDTA plasma (n=8) | 90-102 | 96 |
| Cell culture media (n=8) | 87-103 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 10.88 | 30.87 | 89.76 | 11.78 | 28.38 | 90.90 |
| Standard deviation | 0.70 | 1.49 | 3.46 | 0.64 | 1.26 | 4.67 |
| CV (%) | 6.43 | 4.83 | 3.85 | 5.43 | 4.44 | 5.14 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
