| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.062ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This Viperin Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | RSAD2 |
| Gene ID: | 91543 |
| Uniprot ID: | RSAD2_HUMAN |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Acetylated by HAT1. HAT1-mediated acetylation of Lys-197 in turn recruits UBE4A that stimulates RSAD2 polyubiquitination leading to proteasomal degradation. 'Lys-6'-linked polyubiquitination at Lys-206 leads to RSAD2 protein degradation. |
| Function | Interferon-inducible antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Catalyzes the conversion of cytidine triphosphate (CTP) to 3'-deoxy-3',4'-didehydro-CTP (ddhCTP) via a SAM-dependent radical mechanism. In turn, ddhCTP acts as a chain terminator for the RNA-dependent RNA polymerases from multiple viruses and directly inhibits viral replication. Therefore, inhibits a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), zika virus, and human immunodeficiency virus (HIV-1). Promotes also TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating 'Lys-63'-linked ubiquitination of IRAK1 by TRAF6. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. |
| Protein Name | S-Adenosylmethionine-Dependent Nucleotide Dehydratase Rsad2 Sand Cytomegalovirus-Induced Gene 5 Protein Radical S-Adenosyl Methionine Domain-Containing Protein 2 Virus Inhibitory Protein - Endoplasmic Reticulum-Associated - Interferon-Inducible Viperin |
| Database Links | Reactome: R-HSA-909733 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Peripheral Membrane Protein Cytoplasmic Side Golgi Apparatus Endoplasmic Reticulum Lipid Droplet Mitochondrion Mitochondrion Inner Membrane Mitochondrion Outer Membrane Infection With Human Cytomegalovirus (Hcmv) Causes Relocation To The Golgi Apparatus And To Cytoplasmic Vacuoles Which Also Contain Hcmv Proteins Glycoprotein B And Pp28 Interaction With Human Cytomegalovirus/Hhv-5 Protein Vmia/Ul37 Results In Its Relocalization From The Endoplasmic Reticulum To The Mitochondria |
| Alternative ELISA Names | S-Adenosylmethionine-Dependent Nucleotide Dehydratase Rsad2 ELISA kit Sand ELISA kit Cytomegalovirus-Induced Gene 5 Protein ELISA kit Radical S-Adenosyl Methionine Domain-Containing Protein 2 ELISA kit Virus Inhibitory Protein - Endoplasmic Reticulum-Associated - Interferon-Inducible ELISA kit Viperin ELISA kit RSAD2 ELISA kit CIG5 ELISA kit |
| output |
Information sourced from Uniprot.org
