| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This VEGFR1/FLT1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
FLT1 |
| Gene ID: |
2321 |
| Uniprot ID: |
VGFR1_HUMAN |
| Specificity: |
This kit recognizes Human VEGFR1/FLT1 in samples. No significant cross-reactivity or interference between Human VEGFR1/FLT1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC). |
| Post Translational Modifications | N-glycosylated. Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-1169 is important for interaction with PLCG. Phosphorylation at Tyr-1213 is important for interaction with PIK3R1, PTPN11, GRB2, and PLCG. Phosphorylation at Tyr-1333 is important for endocytosis and for interaction with CBL, NCK1 and CRK. Is probably dephosphorylated by PTPRB. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity.may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 1: Phosphorylates PLCG. Isoform 2: May function as decoy receptor for VEGFA. Isoform 3: May function as decoy receptor for VEGFA. Isoform 4: May function as decoy receptor for VEGFA. Isoform 7: Has a truncated kinase domain.it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion. |
| Protein Name | Vascular Endothelial Growth Factor Receptor 1Vegfr-1Fms-Like Tyrosine Kinase 1Flt-1Tyrosine-Protein Kinase FrtTyrosine-Protein Kinase Receptor FltFltVascular Permeability Factor Receptor |
| Database Links | Reactome: R-HSA-194306Reactome: R-HSA-195399 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinEndosomeAutophosphorylation Promotes Ubiquitination And EndocytosisIsoform 2: SecretedIsoform 3: SecretedIsoform 4: SecretedIsoform 5: CytoplasmIsoform 6: CytoplasmIsoform 7: Cytoplasm |
| Alternative ELISA Names | Vascular Endothelial Growth Factor Receptor 1 ELISA kitVegfr-1 ELISA kitFms-Like Tyrosine Kinase 1 ELISA kitFlt-1 ELISA kitTyrosine-Protein Kinase Frt ELISA kitTyrosine-Protein Kinase Receptor Flt ELISA kitFlt ELISA kitVascular Permeability Factor Receptor ELISA kitFLT1 ELISA kitFLT ELISA kitFRT ELISA kitVEGFR1 ELISA kit |
| Specificity | This kit recognizes Human VEGFR1/FLT1 in samples. No significant cross-reactivity or interference between Human VEGFR1/FLT1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-103 | 95 |
| EDTA plasma (n=8) | 87-98 | 92 |
| Cell culture media (n=8) | 90-104 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 107.21 | 203.54 | 957.45 | 109.81 | 222.50 | 908.10 |
| Standard deviation | 6.06 | 10.42 | 42.99 | 5.98 | 11.75 | 44.22 |
| CV (%) | 5.65 | 5.12 | 4.49 | 5.45 | 5.28 | 4.87 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
