• Human VEGFD (Vascular endothelial growth factor D) CLIA Kit (STJC0000726)

Human VEGFD (Vascular endothelial growth factor D) CLIA Kit (STJC0000726)

SKU:
STJC0000726

£238.50 - £477.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human VEGFD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: VEGFD
Gene ID: 2277
Uniprot ID: VEGFD_HUMAN
Specificity: This kit recognizes Human VEGF-D in samples. No significant cross-reactivity or interference between Human VEGF-D and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.
Post Translational Modifications Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
Function Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
Protein Name Vascular Endothelial Growth Factor D
Vegf-D
C-Fos-Induced Growth Factor
Figf
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-194313
Reactome: R-HSA-195399
Cellular Localisation Secreted
Alternative CLIA Names Vascular Endothelial Growth Factor D CLIA kit
Vegf-D CLIA kit
C-Fos-Induced Growth Factor CLIA kit
Figf CLIA kit
VEGFD CLIA kit
FIGF CLIA kit
Specificity This kit recognizes Human VEGF-D in samples. No significant cross-reactivity or interference between Human VEGF-D and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 101-114 107
EDTA plasma(n=8) 90-101 96
Cell culture media(n=8) 85-97 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 47.70 143.18 386.00 45.77 141.57 380.45
Standard deviation 3.91 13.20 27.25 4.51 16.21 35.88
CV (%) 8.20 9.22 7.06 9.85 11.45 9.43
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance