| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This VEGFB Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
VEGFB |
| Gene ID: |
7423 |
| Uniprot ID: |
VEGFB_HUMAN |
| Specificity: |
This kit recognizes Human VEGF-B in samples. No significant cross-reactivity or interference between Human VEGF-B and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | VEGF-B186 is O-glycosylated. |
| Function | Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. |
| Protein Name | Vascular Endothelial Growth Factor BVegf-BVegf-Related FactorVrf |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-194313Reactome: R-HSA-195399 |
| Cellular Localisation | SecretedSecreted But Remains Associated To Cells Or To The Extracellular Matrix Unless Released By Heparin |
| Alternative ELISA Names | Vascular Endothelial Growth Factor B ELISA kitVegf-B ELISA kitVegf-Related Factor ELISA kitVrf ELISA kitVEGFB ELISA kitVRF ELISA kit |
| Specificity | This kit recognizes Human VEGF-B in samples. No significant cross-reactivity or interference between Human VEGF-B and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-101 | 94 |
| EDTA plasma (n=8) | 93-105 | 98 |
| Cell culture media (n=8) | 92-107 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.07 | 102.94 | 400.36 | 51.34 | 106.20 | 365.76 |
| Standard deviation | 2.75 | 4.67 | 13.05 | 3.14 | 4.59 | 16.06 |
| CV (%) | 5.38 | 4.54 | 3.26 | 6.12 | 4.32 | 4.39 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
