• Human VCP (Valosin Containing Protein) Sandwich ELISA Kit Ready-To-Use (STJE0019376)

Human VCP (Valosin Containing Protein) Sandwich ELISA Kit Ready-To-Use (STJE0019376)

SKU:
STJE0019376

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.122ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This VCP Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: VCP
Gene ID: 7415
Uniprot ID: TERA_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity
Post Translational Modifications Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation. Phosphorylated in mitotic cells. ISGylated. Methylation at Lys-315 catalyzed by VCPKMT is increased in the presence of ASPSCR1. Lys-315 methylation may decrease ATPase activity.
Function Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Involved in clearance process by mediating G3BP1 extraction from stress granules. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Together with SPRTN metalloprotease, involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis. Involved in interstrand cross-link repair in response to replication stress by mediating unloading of the ubiquitinated CMG helicase complex. Mediates extraction of PARP1 trapped to chromatin: recognizes and binds ubiquitinated PARP1 and promotes its removal. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy. Acts as a negative regulator of type I interferon production by interacting with RIGI: interaction takes place when RIGI is ubiquitinated via 'Lys-63'-linked ubiquitin on its CARD domains, leading to recruit RNF125 and promote ubiquitination and degradation of RIGI. May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation. May more particularly play a role in caveolins sorting in cells. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.
Protein Name Transitional Endoplasmic Reticulum Atpase
Ter Atpase
15s Mg(2+-Atpase P97 Subunit
Valosin-Containing Protein
Vcp
Database Links Reactome: R-HSA-110320
Reactome: R-HSA-3371511
Reactome: R-HSA-382556
Reactome: R-HSA-532668
Reactome: R-HSA-5358346
Reactome: R-HSA-5362768
Reactome: R-HSA-5678895
Reactome: R-HSA-5689877
Reactome: R-HSA-5689896
Reactome: R-HSA-6798695
Reactome: R-HSA-8866654
Reactome: R-HSA-8876725
Reactome: R-HSA-8951664
Reactome: R-HSA-9013407
Reactome: R-HSA-9646399
Reactome: R-HSA-9678110
Reactome: R-HSA-9694614
Reactome: R-HSA-9755511
Cellular Localisation Cytoplasm
Cytosol
Endoplasmic Reticulum
Nucleus
Stress Granule
Present In The Neuronal Hyaline Inclusion Bodies Specifically Found In Motor Neurons From Amyotrophic Lateral Sclerosis Patients
Present In The Lewy Bodies Specifically Found In Neurons From Parkinson Disease Patients
Recruited To The Cytoplasmic Surface Of The Endoplasmic Reticulum Via Interaction With Amfr/Gp78
Following Dna Double-Strand Breaks
Recruited To The Sites Of Damage
Recruited To Stalled Replication Forks Via Interaction With Sprtn
Recruited To Damaged Lysosomes Decorated With K48-Linked Ubiquitin Chains
Colocalizes With Tia1
Zfand1 And G3bp1 In Cytoplasmic Stress Granules (Sgs) In Response To Arsenite-Induced Stress Treatment
Alternative ELISA Names Transitional Endoplasmic Reticulum Atpase ELISA kit
Ter Atpase ELISA kit
15s Mg(2+-Atpase P97 Subunit ELISA kit
Valosin-Containing Protein ELISA kit
Vcp ELISA kit
VCP ELISA kit
HEL-220 ELISA kit
HEL-S-70 ELISA kit
output

Information sourced from Uniprot.org

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