Human TTR (Transthyretin) Sandwich ELISA (STJE0019795)

SKU:
STJE0019795

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.14ng/mL
Detection Limit: 0.23~15ng/mL
Short Description: This TTR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TTR
Gene ID: 7276
Uniprot ID: TTHY_HUMAN
Specificity: This kit recognizes Human TTR in samples. No significant cross-reactivity or interference between Human TTR and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
Post Translational Modifications Not glycosylated under normal conditions. Following unfolding, caused for example by variant AMYL-TTR 'Gly-38', the cryptic Asn-118 site is exposed and glycosylated by STT3B-containing OST complex, leading to its degradation by the ER-associated degradation (ERAD) pathway. Sulfonation of the reactive cysteine Cys-30 enhances the stability of the native conformation of TTR, avoiding misassembly of the protein leading to amyloid formation.
Function Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Protein Name Transthyretin
Attr
Prealbumin
Tbpa
Database Links Reactome: R-HSA-2453864
Reactome: R-HSA-2453902
Reactome: R-HSA-3000171
Reactome: R-HSA-6798695
Reactome: R-HSA-975634
Reactome: R-HSA-977225
Cellular Localisation Secreted
Cytoplasm
Alternative ELISA Names Transthyretin ELISA kit
Attr ELISA kit
Prealbumin ELISA kit
Tbpa ELISA kit
TTR ELISA kit
PALB ELISA kit
Specificity This kit recognizes Human TTR in samples. No significant cross-reactivity or interference between Human TTR and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-105 97
EDTA plasma (n=8) 86-97 91
Cell culture media (n=8) 85-96 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.82 2.32 5.57 0.75 2.16 5.49
Standard deviation 0.04 0.13 0.19 0.04 0.12 0.21
CV (%) 5.45 5.70 3.45 5.20 5.50 3.86
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance