| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This TTN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
TTN |
| Gene ID: |
7273 |
| Uniprot ID: |
TITIN_HUMAN |
| Specificity: |
This kit recognizes Human TTN in samples. No significant cross-reactivity or interference between Human TTN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Autophosphorylated. |
| Function | Key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase. |
| Protein Name | TitinConnectinRhabdomyosarcoma Antigen Mu-Rms-40.14 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-390522 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative ELISA Names | Titin ELISA kitConnectin ELISA kitRhabdomyosarcoma Antigen Mu-Rms-40.14 ELISA kitTTN ELISA kit |
| Specificity | This kit recognizes Human TTN in samples. No significant cross-reactivity or interference between Human TTN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-105 | 100 |
| EDTA plasma (n=8) | 88-101 | 95 |
| Cell culture media (n=8) | 88-103 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.46 | 1.13 | 4.55 | 0.51 | 1.13 | 4.84 |
| Standard deviation | 0.03 | 0.06 | 0.22 | 0.04 | 0.06 | 0.25 |
| CV (%) | 6.52 | 5.31 | 4.84 | 7.84 | 5.31 | 5.17 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
