| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.051ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This TRPM1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids.. |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
TRPM1 |
| Gene ID: |
4308 |
| Uniprot ID: |
TRPM1_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in the retina where it localizes to the outer plexiform layer. Specifically, it is expressed in retinal bipolar cells (BPCs) of the ON subtype. Highly expressed in benign melanocytic nevi and diffusely expressed in various in situ melanomas, but not detected in melanoma metastases. Also expressed in melanocytes and pigmented metastatic melanoma cell lines. In melanocytes expression appears to be regulated at the level of transcription and mRNA processing. |
| Post Translational Modifications | |
| Function | Constitutively open nonselective divalent cation-conducting channels which mediate the influx of Ca(2+), Mg(2+), Mn(2+), Ba(2+), and Ni(2+) into the cytoplasm, leading to membrane depolarization. Impermeable to zinc ions. In addition, forms heteromultimeric ion channels with TRPM3 which are permeable for calcium and zinc ions. Plays an essential role for the depolarizing photoresponse of retinal ON bipolar cells. In the dark, tonic release of glutamate activates the G-protein coupled receptor for glutamate, GRM6, its activation induces the release of G(o) protein and the beta-gamma G protein dimer. Both subunits can interact and inactivate the TRPM1 channel. A light onset, induces decrease in glutamate release and deactivation of GRM6 leading to channel opening and membrane depolarization. May play a role in metastasis suppression. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily M Member 1Long Transient Receptor Potential Channel 1Ltrpc1Melastatin-1 |
| Database Links | Reactome: R-HSA-3295583Reactome: R-HSA-9824594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEndoplasmic Reticulum MembraneCell ProjectionAxonIn On-Bipolar Cells Is Intracellular And Located Predominantly In The ErAnd Not At The Plasma Membrane Or Golgi Apparatus |
| Alternative ELISA Names | Transient Receptor Potential Cation Channel Subfamily M Member 1 ELISA kitLong Transient Receptor Potential Channel 1 ELISA kitLtrpc1 ELISA kitMelastatin-1 ELISA kitTRPM1 ELISA kitLTRPC1 ELISA kitMLSN ELISA kitMLSN1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
