• Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) Sandwich ELISA (STJE0003089)
  • Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) Sandwich ELISA (STJE0003089)

Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) Sandwich ELISA (STJE0003089)

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STJE0003089

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This TRAIL/TNFSF10 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TNFSF10
Gene ID: 8743
Uniprot ID: TNF10_HUMAN
Specificity: This kit recognizes Human TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Human TRAIL/TNFSF10 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Tyrosine phosphorylated by PKDCC/VLK.
Function Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Protein Name Tumor Necrosis Factor Ligand Superfamily Member 10
Apo-2 Ligand
Apo-2l
Tnf-Related Apoptosis-Inducing Ligand
Protein Trail
Cd Antigen Cd253
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-69416
Reactome: R-HSA-75158
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Secreted
Exists Both As Membrane-Bound And Soluble Form
Alternative ELISA Names Tumor Necrosis Factor Ligand Superfamily Member 10 ELISA kit
Apo-2 Ligand ELISA kit
Apo-2l ELISA kit
Tnf-Related Apoptosis-Inducing Ligand ELISA kit
Protein Trail ELISA kit
Cd Antigen Cd253 ELISA kit
TNFSF10 ELISA kit
APO2L ELISA kit
TRAIL ELISA kit
Specificity This kit recognizes Human TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Human TRAIL/TNFSF10 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 92
EDTA plasma (n=8) 87-101 93
Cell culture media (n=8) 96-108 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 54.98 160.57 492.42 57.97 153.76 484.84
Standard deviation 3.18 8.49 20.83 2.96 7.70 24.10
CV (%) 5.78 5.29 4.23 5.11 5.01 4.97
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance