| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
29pg/mL |
| Detection Limit: |
78.1-5000pg/mL |
| Short Description: |
This TRAF3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
TRAF3 |
| Gene ID: |
7187 |
| Uniprot ID: |
TRAF3_HUMAN |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. 'Lys-48'-linked polyubiquitinated form is deubiquitinated by OTUD7B, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling. 'Lys-63'-linked ubiquitination can be mediated by TRIM35. Deubiquitinated by OTUB1, OTUB2 and OTUD5. Undergoes 'Lys-63'-linked deubiquitination by MYSM1 to terminate the pattern-recognition receptors/PRRs pathways. Undergoes also 'Lys-29'-linked ubiquitination on Cys-56 and Cys-124 by NEDD4L.leading to increased 'Lys-48'- and 'Lys-63'-linked ubiquitination as well as increased binding to TBK1. TLR4 signals emanating from bacteria containing vesicles trigger 'Lys-33'-linked polyubiquitination that promotes the assembly of the exocyst complex thereby connecting innate immune signaling to the cellular trafficking apparatus. Deubiquitinated by USP25 during viral infection, leading to TRAF3 stabilization and type I interferon production. Ubiquitinated at Lys-329 by the SCF(FBXL2) complex, leading to its degradation by the proteasome. 'Lys-63'-linked ubiquitination by FBXO11 in a NEDD8-dependent manner promotes the amplification of IFN-I signaling. (Microbial infection) Cleaved by enterovirus D68 protease 2A.leading to inhibition of NF-kappa-B or IFN-beta triggered by TRAF3. |
| Function | Cytoplasmic E3 ubiquitin ligase that regulates various signaling pathways, such as the NF-kappa-B, mitogen-activated protein kinase (MAPK) and interferon regulatory factor (IRF) pathways, and thus controls a lot of biological processes in both immune and non-immune cell types. In TLR and RLR signaling pathways, acts as an E3 ubiquitin ligase promoting the synthesis of 'Lys-63'-linked polyubiquitin chains on several substrates such as ASC that lead to the activation of the type I interferon response or the inflammasome. Following the activation of certain TLRs such as TLR4, acts as a negative NF-kappa-B regulator, possibly to avoid unregulated inflammatory response, and its degradation via 'Lys-48'-linked polyubiquitination is required for MAPK activation and production of inflammatory cytokines. Alternatively, when TLR4 orchestrates bacterial expulsion, TRAF3 undergoes 'Lys-33'-linked polyubiquitination and subsequently binds to RALGDS, mobilizing the exocyst complex to rapidly expel intracellular bacteria back for clearance. Acts also as a constitutive negative regulator of the alternative NF-kappa-B pathway, which controls B-cell survival and lymphoid organ development. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. |
| Protein Name | Tnf Receptor-Associated Factor 3Cd40 Receptor-Associated Factor 1Craf1Cd40-Binding ProteinCd40bpLmp1-Associated Protein 1Lap1Ring-Type E3 Ubiquitin Transferase Traf3 |
| Database Links | Reactome: R-HSA-5602571Reactome: R-HSA-5668541Reactome: R-HSA-5676594Reactome: R-HSA-5689896Reactome: R-HSA-9013973Reactome: R-HSA-918233Reactome: R-HSA-936440Reactome: R-HSA-936964Reactome: R-HSA-9692916Reactome: R-HSA-9705671 |
| Cellular Localisation | CytoplasmEndosomeMitochondrionUndergoes Endocytosis Together With Tlr4 Upon Lps SignalingCo-Localized To Mitochondria With Trim35 |
| Alternative ELISA Names | Tnf Receptor-Associated Factor 3 ELISA kitCd40 Receptor-Associated Factor 1 ELISA kitCraf1 ELISA kitCd40-Binding Protein ELISA kitCd40bp ELISA kitLmp1-Associated Protein 1 ELISA kitLap1 ELISA kitRing-Type E3 Ubiquitin Transferase Traf3 ELISA kitTRAF3 ELISA kitCAP-1 ELISA kitCRAF1 ELISA kitTRAFAMN ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
