• Human TNNT1 (Troponin T, slow skeletal muscle) Sandwich ELISA (STJE0003022)

Human TNNT1 (Troponin T, slow skeletal muscle) Sandwich ELISA (STJE0003022)

SKU:
STJE0003022

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This TNNT1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TNNT1
Gene ID: 7138
Uniprot ID: TNNT1_HUMAN
Specificity: This kit recognizes Human TNNT1 in samples. No significant cross-reactivity or interference between Human TNNT1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Troponin T - Slow Skeletal Muscle
Tnts
Slow Skeletal Muscle Troponin T
Stnt
Database Links Reactome: R-HSA-390522
Alternative ELISA Names Troponin T - Slow Skeletal Muscle ELISA kit
Tnts ELISA kit
Slow Skeletal Muscle Troponin T ELISA kit
Stnt ELISA kit
TNNT1 ELISA kit
TNT ELISA kit
Specificity This kit recognizes Human TNNT1 in samples. No significant cross-reactivity or interference between Human TNNT1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-100 95
EDTA plasma (n=8) 90-103 95
Cell culture media (n=8) 92-109 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 51.63 90.62 483.83 49.04 96.14 444.11
Standard deviation 2.82 3.63 26.37 2.95 4.57 17.19
CV (%) 5.46 4.01 5.45 6.02 4.75 3.87
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance