• Human TNK2 (Tyrosine Kinase, Non Receptor 2) Sandwich ELISA Kit Ready-To-Use (STJE0019092)

Human TNK2 (Tyrosine Kinase, Non Receptor 2) Sandwich ELISA Kit Ready-To-Use (STJE0019092)

SKU:
STJE0019092

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.058ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This TNK2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: TNK2
Gene ID: 10188
Uniprot ID: ACK1_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.
Post Translational Modifications Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC and is observed during association with clathrin-coated pits. Polyubiquitinated by NEDD4 and NEDD4L. Degradation can be induced by EGF and is lysosome-dependent.
Function Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.
Protein Name Activated Cdc42 Kinase 1
Ack-1
Tyrosine Kinase Non-Receptor Protein 2
Database Links
Cellular Localisation Cell Membrane
Nucleus
Endosome
Cell Junction
Adherens Junction
Cytoplasmic Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Vesicle
Clathrin-Coated Vesicle
Membrane
Clathrin-Coated Pit
Cytoplasm
Perinuclear Region
Cytosol
The Tyr-284 Phosphorylated Form Is Found Both In The Membrane And Nucleus
Co-Localizes With Egfr On Endosomes
Nuclear Translocation Is Cdc42-Dependent
Detected In Long Filamentous Cytosolic Structures Where It Co-Localizes With Ctps1
Alternative ELISA Names Activated Cdc42 Kinase 1 ELISA kit
Ack-1 ELISA kit
Tyrosine Kinase Non-Receptor Protein 2 ELISA kit
TNK2 ELISA kit
ACK1 ELISA kit
output

Information sourced from Uniprot.org

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