| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
26.15 pg/mL |
| Detection Limit: |
78.13-5000 pg/mL |
| Short Description: |
This TNC Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
TNC |
| Gene ID: |
3371 |
| Uniprot ID: |
TENA_HUMAN |
| Specificity: |
This kit recognizes Human TNC in samples. No significant cross-reactivity or interference between Human TNC and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Detected in fibroblasts (at protein level). |
| Function | Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells. |
| Protein Name | TenascinTnCytotactinGmemGp 150-225Glioma-Associated-Extracellular Matrix AntigenHexabrachionJiMyotendinous AntigenNeuronectinTenascin-CTn-C |
| Database Links | Reactome: R-HSA-216083Reactome: R-HSA-3000170Reactome: R-HSA-3000178Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative ELISA Names | Tenascin ELISA kitTn ELISA kitCytotactin ELISA kitGmem ELISA kitGp 150-225 ELISA kitGlioma-Associated-Extracellular Matrix Antigen ELISA kitHexabrachion ELISA kitJi ELISA kitMyotendinous Antigen ELISA kitNeuronectin ELISA kitTenascin-C ELISA kitTn-C ELISA kitTNC ELISA kitHXB ELISA kit |
| Specificity | This kit recognizes Human TNC in samples. No significant cross-reactivity or interference between Human TNC and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-99 | 91 |
| EDTA plasma (n=8) | 84-96 | 91 |
| Cell culture media (n=8) | 96-112 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 273.16 | 598.05 | 2431.85 | 251.78 | 555.12 | 2379.10 |
| Standard deviation | 16.14 | 31.64 | 117.70 | 15.43 | 23.98 | 113.48 |
| CV (%) | 5.91 | 5.29 | 4.84 | 6.13 | 4.32 | 4.77 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
