| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.135ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This TLR5 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
TLR5 |
| Gene ID: |
7100 |
| Uniprot ID: |
TLR5_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Highly expressed on the basolateral surface of intestinal epithelia. Expressed also in other cells such as lung epithelial cells. |
| Post Translational Modifications | Phosphorylated at Ser-805 by PKD/PRKD1.phosphorylation induces the production of inflammatory cytokines. Phosphorylated at Tyr-798 upon flagellin binding.required for signaling. |
| Function | Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life. |
| Protein Name | Toll-Like Receptor 5Toll/Interleukin-1 Receptor-Like Protein 3 |
| Database Links | Reactome: R-HSA-168176Reactome: R-HSA-5602680Reactome: R-HSA-5603037Reactome: R-HSA-975871 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | Toll-Like Receptor 5 ELISA kitToll/Interleukin-1 Receptor-Like Protein 3 ELISA kitTLR5 ELISA kitTIL3 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
