| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human TIMP4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
TIMP4 |
| Gene ID: |
7079 |
| Uniprot ID: |
TIMP4_HUMAN |
| Specificity: |
This kit recognizes Human TIMP-4 in samples. No significant cross-reactivity or interference between Human TIMP-4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Abundant in heart and present at low levels in many other tissues. |
| Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. |
| Protein Name | Metalloproteinase Inhibitor 4Tissue Inhibitor Of Metalloproteinases 4Timp-4 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Metalloproteinase Inhibitor 4 CLIA kitTissue Inhibitor Of Metalloproteinases 4 CLIA kitTimp-4 CLIA kitTIMP4 CLIA kit |
| Specificity | This kit recognizes Human TIMP-4 in samples. No significant cross-reactivity or interference between Human TIMP-4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 97-109 | 103 |
| EDTA plasma(n=8) | 97-115 | 105 |
| Cell culture media(n=8) | 91-108 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 45.57 | 112.76 | 380.28 | 47.95 | 118.58 | 384.23 |
| Standard deviation | 4.48 | 10.77 | 23.69 | 5.32 | 9.45 | 39.96 |
| CV (%) | 9.83 | 9.55 | 6.23 | 11.09 | 7.97 | 10.40 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
