| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human TIMP2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
TIMP2 |
| Gene ID: |
7077 |
| Uniprot ID: |
TIMP2_HUMAN |
| Specificity: |
This kit recognizes Human TIMP-2 in samples. No significant cross-reactivity or interference between Human TIMP-2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The activity of TIMP2 is dependent on the presence of disulfide bonds. |
| Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. |
| Protein Name | Metalloproteinase Inhibitor 2Csc-21kTissue Inhibitor Of Metalloproteinases 2Timp-2 |
| Database Links | Reactome: R-HSA-1592389Reactome: R-HSA-6798695 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Metalloproteinase Inhibitor 2 CLIA kitCsc-21k CLIA kitTissue Inhibitor Of Metalloproteinases 2 CLIA kitTimp-2 CLIA kitTIMP2 CLIA kit |
| Specificity | This kit recognizes Human TIMP-2 in samples. No significant cross-reactivity or interference between Human TIMP-2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-115 | 106 |
| EDTA plasma(n=8) | 92-104 | 98 |
| Cell culture media(n=8) | 97-111 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 107.39 | 196.54 | 787.38 | 103.57 | 214.28 | 763.36 |
| Standard deviation | 9.42 | 18.08 | 65.12 | 8.69 | 18.32 | 84.89 |
| CV (%) | 8.77 | 9.20 | 8.27 | 8.39 | 8.55 | 11.12 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
