• Human THBD (Thrombomodulin) CLIA Kit (STJC0000131)

Human THBD (Thrombomodulin) CLIA Kit (STJC0000131)

SKU:
STJC0000131

€298.13 - €596.88
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human THBD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: THBD
Gene ID: 7056
Uniprot ID: TRBM_HUMAN
Specificity: This kit recognizes Human TM in samples. No significant cross-reactivity or interference between Human TM and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Endothelial cells are unique in synthesizing thrombomodulin.
Post Translational Modifications N-glycosylated. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Function Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
Protein Name Thrombomodulin
Tm
Fetomodulin
Cd Antigen Cd141
Database Links Reactome: R-HSA-140875
Reactome: R-HSA-202733
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative CLIA Names Thrombomodulin CLIA kit
Tm CLIA kit
Fetomodulin CLIA kit
Cd Antigen Cd141 CLIA kit
THBD CLIA kit
THRM CLIA kit
Specificity This kit recognizes Human TM in samples. No significant cross-reactivity or interference between Human TM and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-104 97
EDTA plasma(n=8) 91-103 97
Cell culture media(n=8) 84-95 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 48.37 102.84 387.49 50.26 109.78 366.01
Standard deviation 3.96 8.64 33.98 5.71 9.22 28.04
CV (%) 8.19 8.40 8.77 11.36 8.40 7.66
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance