• Human TGF-beta 1 (C33S) protein (Active Recombinant) (N-His) (STJP000936)
  • Human TGF-beta 1 (C33S) protein (Active Recombinant) (N-His) (STJP000936)
  • Human TGF-beta 1 (C33S) protein (Active Recombinant) (N-His) (STJP000936)
  • Human TGF-beta 1 (C33S) protein (Active Recombinant) (N-His) (STJP000936)
  • Human TGF-beta 1 (C33S) protein (Active Recombinant) (N-His) (STJP000936)
  • Human TGF-beta 1 (C33S) protein (Active Recombinant) (N-His) (STJP000936)
  • Human TGF-beta 1 (C33S) protein (Active Recombinant) (N-His) (STJP000936)
  • Human TGF-beta 1 (C33S) protein (Active Recombinant) (N-His) (STJP000936)

Human TGF-beta 1 (C33S) protein (Active Recombinant) (N-His) (STJP000936)

SKU:
STJP000936

Current Stock:
Host: HEK293 cells
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Active Recombinant-Human TGF-beta 1 (C33S)-N-His protein was developed from hek293 cells and has a target region of N-His. For use in research applications.
Formulation: Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Immunoreactivity: Measured by its ability to inhibit cell proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2 ng/mL, corresponding to a specific activity of 5.0×105~2.0×106 units/mg.2. Measured by its binding ability in a fun
Gene Symbol: TGFB1
Gene ID: 7040
Uniprot ID: TGFB1_HUMAN
Immunogen Region: Leu30-Ser390C33S
Immunogen: Active Recombinant Human Latent TGF-beta 1 (C33S) Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu30-Ser390 (C33S) ) of human TGF-beta 1 (Accession #NP_000651.3) fused with an 8×His tag at the N-ter
Tissue Specificity Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage.
Post Translational Modifications Transforming growth factor beta-1 proprotein: The precursor proprotein is cleaved in the Golgi apparatus by FURIN to form Transforming growth factor beta-1 (TGF-beta-1) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-1 inactive. Latency-associated peptide: N-glycosylated. Deglycosylation leads to activation of Transforming growth factor beta-1 (TGF-beta-1).mechanisms triggering deglycosylation-driven activation of TGF-beta-1 are however unclear.
Function Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. Latency-associated peptide: Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia (Probable). Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1. Transforming growth factor beta-1: Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1. Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules'. Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Positively regulates odontoblastic differentiation in dental papilla cells, via promotion of IPO7-mediated translocation of phosphorylated SMAD2 to the nucleus and subsequent transcription of target genes. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.
Protein Name Transforming Growth Factor Beta-1 Proprotein Cleaved Into - Latency-Associated Peptide
Lap - Transforming Growth Factor Beta-1
Tgf-Beta-1
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-168277
Reactome: R-HSA-202733
Reactome: R-HSA-2129379
Reactome: R-HSA-2173788
Reactome: R-HSA-2173789
Reactome: R-HSA-2173791
Reactome: R-HSA-3000170
Reactome: R-HSA-3000178
Reactome: R-HSA-3304356
Reactome: R-HSA-3642279
Reactome: R-HSA-3645790
Reactome: R-HSA-3656532
Reactome: R-HSA-3656535
Reactome: R-HSA-381340
Reactome: R-HSA-5689603
Reactome: R-HSA-6785807
Reactome: R-HSA-8941855
Reactome: R-HSA-8941858
Reactome: R-HSA-8951936
Cellular Localisation Latency-Associated Peptide: Secreted
Extracellular Space
Extracellular Matrix
Transforming Growth Factor Beta-1: Secreted
Alternative Protein Names Transforming Growth Factor Beta-1 Proprotein Cleaved Into - Latency-Associated Peptide protein
Lap - Transforming Growth Factor Beta-1 protein
Tgf-Beta-1 protein
TGFB1 protein
TGFB protein

Information sourced from Uniprot.org

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