Applications: |
CLIA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
18.75pg/mL |
Detection Limit: |
31.25~2000pg/mL |
Short Description: |
This human TFPI kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
TFPI |
Gene ID: |
7035 |
Uniprot ID: |
TFPI1_HUMAN |
Specificity: |
This kit recognizes Human TFPI in samples. No significant cross-reactivity or interference between Human TFPI and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Tissue Specificity | Mostly in endothelial cells. |
Post Translational Modifications | O-glycosylated. |
Function | Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. |
Protein Name | Tissue Factor Pathway InhibitorTfpiExtrinsic Pathway InhibitorEpiLipoprotein-Associated Coagulation InhibitorLaci |
Database Links | Reactome: R-HSA-140834 |
Cellular Localisation | Isoform Alpha: SecretedIsoform Beta: Microsome MembraneLipid-AnchorGpi-Anchor |
Alternative CLIA Names | Tissue Factor Pathway Inhibitor CLIA kitTfpi CLIA kitExtrinsic Pathway Inhibitor CLIA kitEpi CLIA kitLipoprotein-Associated Coagulation Inhibitor CLIA kitLaci CLIA kitTFPI CLIA kitLACI CLIA kitTFPI1 CLIA kit |
Specificity | This kit recognizes Human TFPI in samples. No significant cross-reactivity or interference between Human TFPI and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 88-99 | 93 |
EDTA plasma(n=8) | 95-109 | 103 |
Cell culture media(n=8) | 97-114 | 105 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 103.02 | 278.52 | 872.74 | 100.06 | 252.51 | 820.70 |
Standard deviation | 12.23 | 29.86 | 69.91 | 9.28 | 24.27 | 89.54 |
CV (%) | 11.87 | 10.72 | 8.01 | 9.27 | 9.61 | 10.91 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance