| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.038ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This TDG Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
TDG |
| Gene ID: |
6996 |
| Uniprot ID: |
TDG_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA. |
| Function | DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. |
| Protein Name | G/T Mismatch-Specific Thymine Dna GlycosylaseThymine-Dna GlycosylaseHtdg |
| Database Links | Reactome: R-HSA-110328Reactome: R-HSA-110329Reactome: R-HSA-110357Reactome: R-HSA-3108214Reactome: R-HSA-5221030 |
| Cellular Localisation | Nucleus |
| Alternative ELISA Names | G/T Mismatch-Specific Thymine Dna Glycosylase ELISA kitThymine-Dna Glycosylase ELISA kitHtdg ELISA kitTDG ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
