| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
35.9 pg/mL |
| Detection Limit: |
125-8000 pg/mL |
| Short Description: |
This sSELL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SELL |
| Gene ID: |
6402 |
| Uniprot ID: |
LYAM1_HUMAN |
| Specificity: |
This kit recognizes Human sSELL in samples. No significant cross-reactivity or interference between Human sSELL and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in B-cell lines and T-lymphocytes. |
| Post Translational Modifications | N-glycosylated. |
| Function | Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. |
| Protein Name | L-SelectinCd62 Antigen-Like Family Member LLeukocyte Adhesion Molecule 1Lam-1Leukocyte Surface Antigen Leu-8Leukocyte-Endothelial Cell Adhesion Molecule 1Lecam1Lymph Node Homing ReceptorTq1Gp90-MelCd Antigen Cd62l |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-202733Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | L-Selectin ELISA kitCd62 Antigen-Like Family Member L ELISA kitLeukocyte Adhesion Molecule 1 ELISA kitLam-1 ELISA kitLeukocyte Surface Antigen Leu-8 ELISA kitLeukocyte-Endothelial Cell Adhesion Molecule 1 ELISA kitLecam1 ELISA kitLymph Node Homing Receptor ELISA kitTq1 ELISA kitGp90-Mel ELISA kitCd Antigen Cd62l ELISA kitSELL ELISA kitLNHR ELISA kitLYAM1 ELISA kit |
| Specificity | This kit recognizes Human sSELL in samples. No significant cross-reactivity or interference between Human sSELL and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-108 | 99 |
| EDTA plasma (n=8) | 88-102 | 95 |
| Cell culture media (n=8) | 95-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 428.81 | 932.30 | 3713.87 | 458.72 | 1018.42 | 4073.67 |
| Standard deviation | 24.40 | 41.30 | 188.66 | 23.95 | 56.73 | 148.69 |
| CV (%) | 5.69 | 4.43 | 5.08 | 5.22 | 5.57 | 3.65 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
