| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
27ng/mL |
| Detection Limit: |
78.1-5000pg/mL |
| Short Description: |
This SPTLC1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
SPTLC1 |
| Gene ID: |
10558 |
| Uniprot ID: |
SPTC1_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Widely expressed. Not detected in small intestine. |
| Post Translational Modifications | Phosphorylation at Tyr-164 inhibits activity and promotes cell survival. |
| Function | Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis. |
| Protein Name | Serine Palmitoyltransferase 1Long Chain Base Biosynthesis Protein 1Lcb 1Serine-Palmitoyl-Coa Transferase 1Spt 1Spt1 |
| Database Links | Reactome: R-HSA-1660661 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane Protein |
| Alternative ELISA Names | Serine Palmitoyltransferase 1 ELISA kitLong Chain Base Biosynthesis Protein 1 ELISA kitLcb 1 ELISA kitSerine-Palmitoyl-Coa Transferase 1 ELISA kitSpt 1 ELISA kitSpt1 ELISA kitSPTLC1 ELISA kitLCB1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
