| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This SPARC Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SPARC |
| Gene ID: |
6678 |
| Uniprot ID: |
SPRC_HUMAN |
| Specificity: |
This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Protein Name | SparcBasement-Membrane Protein 40Bm-40OsteonectinOnSecreted Protein Acidic And Rich In Cysteine |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1251985Reactome: R-HSA-3000178Reactome: R-HSA-3000497 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneIn Or Around The Basement Membrane |
| Alternative ELISA Names | Sparc ELISA kitBasement-Membrane Protein 40 ELISA kitBm-40 ELISA kitOsteonectin ELISA kitOn ELISA kitSecreted Protein Acidic And Rich In Cysteine ELISA kitSPARC ELISA kitON ELISA kit |
| Specificity | This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-101 | 96 |
| EDTA plasma (n=8) | 88-102 | 93 |
| Cell culture media (n=8) | 89-104 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.08 | 1.91 | 8.12 | 1.14 | 1.95 | 7.33 |
| Standard deviation | 0.07 | 0.11 | 0.38 | 0.06 | 0.10 | 0.37 |
| CV (%) | 6.48 | 5.76 | 4.68 | 5.26 | 5.13 | 5.05 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
