• Human SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit (STJE0002728)

Human SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit (STJE0002728)

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Applications: ELISA
Reactivity: Human
Short Description: This SOD1 Competitive ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Detection: Colorimetric
Gene ID: 6647
Sample Type: Serum, Plasma, Cell supernatant
Background This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human SOD1. During the reaction, Human SOD1 in the sample or standard competes with a fixed amount of Human SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human SOD1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human SOD1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Post Translational Modifications Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation. The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. Palmitoylation helps nuclear targeting and decreases catalytic activity. Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity.
Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Database Links Reactome: R-HSA-114608
Cellular Localisation Cytoplasm
Predominantly Cytoplasmic
The Pathogenic Variants Als1 Arg-86 And Ala-94 Gradually Aggregates And Accumulates In Mitochondria
Protein Name Superoxide Dismutase Cu-Zn
Superoxide Dismutase 1
Alternative Names Anti-Superoxide Dismutase Cu-Zn antibody
Anti-Superoxide Dismutase 1 antibody
Anti-Hsod1 antibody
Anti-SOD1 antibody

Information sourced from Uniprot.org

6 months standard