| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human SOCS3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
SOCS3 |
| Gene ID: |
9021 |
| Uniprot ID: |
SOCS3_HUMAN |
| Specificity: |
This kit recognizes Human SOCS3 in samples. No significant cross-reactivity or interference between Human SOCS3 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. |
| Post Translational Modifications | Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1. |
| Function | SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity and regulates IL6 signaling. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. |
| Protein Name | Suppressor Of Cytokine Signaling 3Socs-3Cytokine-Inducible Sh2 Protein 3Cis-3Stat-Induced Stat Inhibitor 3Ssi-3 |
| Database Links | Reactome: R-HSA-1059683Reactome: R-HSA-2586552Reactome: R-HSA-6785807Reactome: R-HSA-877300Reactome: R-HSA-877312Reactome: R-HSA-8849474Reactome: R-HSA-8939242Reactome: R-HSA-8951664Reactome: R-HSA-909733Reactome: R-HSA-912694Reactome: R-HSA-9674555Reactome: R-HSA-9705462Reactome: R-HSA-982772Reactome: R-HSA-983168 |
| Alternative CLIA Names | Suppressor Of Cytokine Signaling 3 CLIA kitSocs-3 CLIA kitCytokine-Inducible Sh2 Protein 3 CLIA kitCis-3 CLIA kitStat-Induced Stat Inhibitor 3 CLIA kitSsi-3 CLIA kitSOCS3 CLIA kitCIS3 CLIA kitSSI3 CLIA kit |
| Specificity | This kit recognizes Human SOCS3 in samples. No significant cross-reactivity or interference between Human SOCS3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 102-115 | 108 |
| EDTA plasma(n=8) | 94-107 | 101 |
| Cell culture media(n=8) | 96-111 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 24.73 | 79.10 | 215.07 | 25.21 | 85.55 | 234.72 |
| Standard deviation | 2.95 | 6.14 | 18.24 | 2.99 | 6.60 | 15.14 |
| CV (%) | 11.93 | 7.76 | 8.48 | 11.86 | 7.71 | 6.45 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
