| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.054ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This SKP1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
SKP1 |
| Gene ID: |
6500 |
| Uniprot ID: |
SKP1_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Undergoes autophagy-mediated degradation in the liver in a time-dependent manner. |
| Function | Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'.the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2. |
| Protein Name | S-Phase Kinase-Associated Protein 1Cyclin-A/Cdk2-Associated Protein P19P19aOrgan Of Corti Protein 2Ocp-2Organ Of Corti Protein IiOcp-IiRna Polymerase Ii Elongation Factor-Like ProteinSiiiTranscription Elongation Factor B Polypeptide 1-LikeP19skp1 |
| Database Links | Reactome: R-HSA-1169091Reactome: R-HSA-1170546Reactome: R-HSA-174113Reactome: R-HSA-180534Reactome: R-HSA-187577Reactome: R-HSA-195253Reactome: R-HSA-202424Reactome: R-HSA-2122947Reactome: R-HSA-2565942Reactome: R-HSA-2644606Reactome: R-HSA-2644607Reactome: R-HSA-2871837Reactome: R-HSA-2894862Reactome: R-HSA-400253Reactome: R-HSA-5607761Reactome: R-HSA-5607764Reactome: R-HSA-5610780Reactome: R-HSA-5610783Reactome: R-HSA-5610785Reactome: R-HSA-5676590Reactome: R-HSA-5684264Reactome: R-HSA-68949Reactome: R-HSA-69231Reactome: R-HSA-8854050Reactome: R-HSA-8939902Reactome: R-HSA-8951664Reactome: R-HSA-9020702Reactome: R-HSA-917937Reactome: R-HSA-9604323Reactome: R-HSA-9708530Reactome: R-HSA-9725371Reactome: R-HSA-9762114Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative ELISA Names | S-Phase Kinase-Associated Protein 1 ELISA kitCyclin-A/Cdk2-Associated Protein P19 ELISA kitP19a ELISA kitOrgan Of Corti Protein 2 ELISA kitOcp-2 ELISA kitOrgan Of Corti Protein Ii ELISA kitOcp-Ii ELISA kitRna Polymerase Ii Elongation Factor-Like Protein ELISA kitSiii ELISA kitTranscription Elongation Factor B Polypeptide 1-Like ELISA kitP19skp1 ELISA kitSKP1 ELISA kitEMC19 ELISA kitOCP2 ELISA kitSKP1A ELISA kitTCEB1L ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
