• Human SIRT4 (Sirtuin 4) Sandwich ELISA Kit Ready-To-Use (STJE0018564)

Human SIRT4 (Sirtuin 4) Sandwich ELISA Kit Ready-To-Use (STJE0018564)

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STJE0018564

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.051ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This SIRT4 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: SIRT4
Gene ID: 23409
Uniprot ID: SIR4_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Tissue Specificity Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus.
Post Translational Modifications
Function Acts as NAD-dependent protein lipoamidase, biotinylase, deacetylase and ADP-ribosyl transferase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Does not seem to deacetylate PC. Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1-PPARA interaction probably through the regulation of NAD(+) levels. Down-regulates insulin secretion.
Protein Name Nad-Dependent Protein Lipoamidase Sirtuin-4 - Mitochondrial
Nad-Dependent Adp-Ribosyltransferase Sirtuin-4
Nad-Dependent Protein Biotinylase Sirtuin-4
Nad-Dependent Protein Deacetylase Sirtuin-4
Regulatory Protein Sir2 Homolog 4
Sir2-Like Protein 4
Database Links Reactome: R-HSA-2151201
Cellular Localisation Mitochondrion Matrix
Alternative ELISA Names Nad-Dependent Protein Lipoamidase Sirtuin-4 - Mitochondrial ELISA kit
Nad-Dependent Adp-Ribosyltransferase Sirtuin-4 ELISA kit
Nad-Dependent Protein Biotinylase Sirtuin-4 ELISA kit
Nad-Dependent Protein Deacetylase Sirtuin-4 ELISA kit
Regulatory Protein Sir2 Homolog 4 ELISA kit
Sir2-Like Protein 4 ELISA kit
SIRT4 ELISA kit
SIR2L4 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance